public read uid=datalibrarian,o=unaffiliated,dc=ecoinformatics,dc=org all uid=shcherbak,o=unaffiliated,dc=ecoinformatics,dc=org read uid=kelly,o=unaffiliated,dc=ecoinformatics,dc=org all Mr. Kevin Kelly Future Farming Systems Research Division, Victorian Department of Environment and Primary Industries (Victoria) Project Leader

Private Bag 1 Ferguson Road Tatura Victoria 3616 Australia

+61 3 5833 5359 kevin.kelly@depi.vic.gov.au Ms. Siobhann McCafferty Institute for Future Environments, Queensland University of Technology Data Librarian

2 George Street Brisbane QLD 4001 Australia

+61 7 3138 0457 siobhann.mccafferty@qut.edu.au Custodian/Steward A study of the impact of the nitrification inhibitors dicyandiamide (DCD) on nitrous oxide emissions from dairy cows urine deposited on pasture was conducted at Terang in south-western Victoria, Australia from August 2009 to April 2010. Nitrous oxide fluxes were measured on four replicates of six treatments. Treatments were; nil applied (no nitrogen added treatment), DCD applied 14 August 2009, urine@1000kg N/ha applied 11 September 2009, urine@1000kg N/ha applied September 2009 plus DCD @ 10kg a.i./ha applied 14 August 2009, urine @ 1000kg N/ha applied 14 August 2009, urine @ 1000kg N/ha applied 14 August plus DCD @ 10 kg a.i./ha applied 14 August 2009. Nitrous oxide flux was measured in TWO replicates of treatments 3,4,5 and 6. Measurement system used automated chambers liked to a Fourier Transform infrared spectometer for gas analysis. Daily flux (g N2O-N/ha/day) was calculated for each chamber. Soil water (surface 0-65 mm) and soil temperature (50mm) was measured continuously in each chamber base. Soil mineral N content of all treatment and replicates was measured (0-100 mm) routinely. Initial site soil physical and chemical status were defined. Standard meteorological information was collected for an on-site weather station. Nitrous oxide N2O Nitrification inhibitors Soil mineral nitrogen Dicyandiamide (DCD) Bovine urine Pasture Dairy Cattle Terang Auto Chambers VIC 0502 0799 anzsrc-for DemoDAIRY, Terang, Victoria, Australia 38 14 S, 142 55 E 142.55 142.55 -38.14 -38.14 1332290672437

Nitrous oxide flux was measured in TWO replicates of treatments 3,4,5 and 6. Flux was measured using 8 automatic enclosure chambers (0.8m*0.8m*0.47m) with air drawn from each chamber in sequence at a flow rate of approximately 3 L/min and passed through a glass cell mounted in a Fourier Transform Infrared spectrometer (FTIR), all air was recycled to chambers. Instantaneous flux was calculated for one chamber every 30 minute period from an 18 minute closure period. Daily flux (g N2O-N/ha/day) was calculated from 6 measurement periods on each chamber each day as an arithmetic mean. Each chamber had to fixed bases with the chamber moved very week. Soil water (surface 0-65 mm) and soil temperature (50mm) was measured in each chamber base. Soil mineral N content of al treatment and replicates was measured (0-100 mm) routinely.

FTIR - standard Bomem FTLA2000 with KBr optics, multipass White cell and InSb detector, 32 m pathlength gas cell (approximate volume 4 L)

Surface soil water content (0-65 mm), two probes per chamber base, logged every 30 minutes (averag of 10 minute readings)

Theta-Probes Delta T MK2x

Soil temperature - logged every 30 minutes (averag of reading every 10 minutes)

Type-T thermocouples

Standard measurement weather station

MEA - Australia August 2009 - April 2010 Experimental design (6 treatments by 4 replicates) * Treatment 1 - nil applied, * Treatment 2 - DCD applied 14 August 2009 * Treatment 3 - urine @ 1000 kg N/ha applied 11 September 2009 * Treatment 4 - urine @ 1000 kg N/ha applied 11 September 2009 plus DCD @ 10 kg a.i./ha applied 14 August 2009 * Treatment 5 - urine @ 1000 kg N/ha applied 14 August 2009 * Treatment 6 - urine @ 1000 kg N/ha applied 14 August 2009 plus DCD @ 10 kg a.i./ha applied 14 August 2009. Weather - soil temperature, soil water, nitrous oxide flux all chambers (8) Expt 1 part 1 Terang data weather and flux data expt 1 part 1.csv column , ecogrid://knb/kelly.23.1 uid=datalibrarian,o=unaffiliated,dc=ecoinformatics,dc=org all uid=shcherbak,o=unaffiliated,dc=ecoinformatics,dc=org read uid=kelly,o=unaffiliated,dc=ecoinformatics,dc=org all Date DD-MM-YYYY DD-MM-YYYY Min Temperature Minimum Temperature - from 9 am previous day to 9 am date recorded - Times are Eastern Australian Standard Time celsius natural Max Temperature Maximum temperature - 9 am previous day to 9 am date of record - Time Eastern Australian Standard Time celsius natural Solar radiation Solar radition (MJ/m^2) megajoulePersquaremeter natural Rainfall Rainfall (mm/day) mm/day natural ETo (evapotranspiration) ETo calculed based on FAO 56 mm/day natural Soil temperature (T3 rep 1) Soil temperature (50 mm) celsius natural Soil water (T3 r1) Soil water - surface volumetric cubicmillimeterPercubicmillimeter natural Nitrous oxide flux (T3 r1) Nitrous oxide flux (g N2O-N/ha/day) gramsNitrogenperHectareperDay natural Soil temperature (T3 r2) Soil temperature (50 mm) celsius natural Soil water (T3 r2) Soil water surface - volumetric cubicmillimeterPercubicmillimeter natural Nitrous oxide flux (T3 r2) Nitrous oxide flux (g N2O-N/ha/day) gramsNitrogenperHectareperDay natural Soil temperature (T4 r1) Soil temperature (50 mm) celsius natural Soil water (T4 r1) Soil water surface - volumetric cubicmillimeterPercubicmillimeter natural Nitrous oxide flux (T4 r1) Nitrous oxide flux (g N2O-N/ha/day) gramsNitrogenperHectareperDay natural Soil temperature (T4 r2) Soil temperature (50 mm) celsius natural Soil water (T4 r2) Soil water surface - volumetric cubicmillimeterPercubicmillimeter natural Nitrous oxide flux (T4 r2) Nitrous oxide flux (g N2O-N/ha/day) gramsNitrogenperHectareperDay natural Soil temperature (T5 r1) Soil temperature (50 mm) celsius natural Soil water (T5 r1) Soil water surface - volumetric cubicmillimeterPercubicmillimeter natural Nitrous oxide flux (T5 r1) Nitrous oxide flux (g N2O-N/ha/day) gramsNitrogenperHectareperDay natural Soil temperature (T5 r2) Soil temperature (50 mm) celsius natural Soil water (T5 r2) Soil water surface - volumetric cubicmillimeterPercubicmillimeter natural Nitrous oxide flux (T5 r2) Nitrous oxide flux (g N2O-N/ha/day) gramsNitrogenperHectareperDay natural Soil temperature (T6 r1) Soil temperature (50 mm) celsius natural Soil water (T6 r1) Soil water surface - volumetric cubicmillimeterPercubicmillimeter natural Nitrous oxide flux (T6 r1) Nitrous oxide flux (g N2O-N/ha/day) gramsNitrogenperHectareperDay natural Soil temperature (T6 r2) Soil temperature (50 mm) celsius natural Soil water (T6 r2) Soil water surface - volumetric cubicmillimeterPercubicmillimeter natural Nitrous oxide flux (T6 r2) Nitrous oxide flux (g N2O-N/ha/ay) gramsNitrogenperHectareperDay natural Soil mineral N content - 6 treatments - expt 1 part 1 Terang soil mineral N expt 1.1.csv column , ecogrid://knb/kelly.26.1 uid=datalibrarian,o=unaffiliated,dc=ecoinformatics,dc=org all uid=shcherbak,o=unaffiliated,dc=ecoinformatics,dc=org read uid=kelly,o=unaffiliated,dc=ecoinformatics,dc=org all Date DD-MM-YYYY DD-MM-YY Treatment 1 (Soil ammomium - N) Soil ammonium - N (mg NH4-N/kg of soil) milligramPerkilogramofsoil natural Treatment 2 (Soil ammonium - N) Soil ammonium - N (mg NH4-N/kg of soil) milligramPerkilogramofsoil natural Treatment 3 (Soil ammonium - N) Soil ammonium - N (mg NH4-N /kg of soil) milligramPerkilogramofsoil natural Treatment 4 (soil ammonium - N) Soil ammonium - N (mg NH4-N/kg of soil) milligramPerkilogramofsoil natural Treatment 5 (soil ammonium - N) Soil ammonium - N (mg NH4-N/kg of soil) milligramPerkilogramofsoil natural Treatment 6 (Soil ammonium - N) Soil ammonium - N (mg NH4-N/kg of soil) milligramPerkilogramofsoil natural l.s.d. (P=0.05) Ammonium-N Least significant difference n.s. = not significant milligramPerkilogramofsoil natural Treatment 1 (Soil nitrate-N) Soil nitrate - N (mg NO3-N/kg of soil) milligramPerkilogramofsoil natural Treatment 2 (soil nitrate - N) Soil nitrate - N (mg NO3-N/kg of soil) milligramPerkilogramofsoil natural Treatment 3 (Soil nitrate - N) Soil nitrate - N (mg NO3-N/kg of soil) milligramPerkilogramofsoil natural Treatment 4 (Soil nitrate - N) Soil nitrate - N (mg NO3-N/kg of soil) milligramPerkilogramofsoil natural Treatment 5 (Soil nitrate - N) Soil nitrate - N (mg NO3-N/kg of soil) milligramPerkilogramofsoil natural Treatment 6 (Soil nitrate - N) Soil nitrate - N (mg NO3-N/kg of soil) milligramPerkilogramofsoil natural l.s.d. (P=0.05) Nitrate - N least signifiacnt difference (P=0.05) n.s. = not significant milligramPerkilogramofsoil natural megajoulePersquaremeter mm/day cubicmm/cubicmm Soil water content (volumetric) Nitrous oxide flux g N2O-N/ha/d mm^3/mm^3 Soil mineral nitrogen