public read uid=datalibrarian,o=unaffiliated,dc=ecoinformatics,dc=org all uid=shcherbak,o=unaffiliated,dc=ecoinformatics,dc=org read uid=kelly,o=unaffiliated,dc=ecoinformatics,dc=org all Mr. Kevin Kelly Future Farming Systems Research Division, Victorian Department of Environment and Primary Industries (Victoria) Project Leader

Private Bag 1 Ferguson Road Tatura Victoria 3616 Australia

+61 3 5833 5359 kevin.kelly@depi.vic.gov.au 1332290672437 Content Provider Ms. Siobhann McCafferty Institute for Future Environments, Queensland University of Technology Data Librarian

2 George Street Brisbane QLD 4001 Australia

+61 7 3138 0457 siobhann.mccafferty@qut.edu.au Custodian/Steward A study of the impact of the nitrification inhibitors dicyandiamide (DCD) on nitrous oxide emissions from dairy cows urine deposited on pasture was conducted at Terang in south-western Victoria, Australia from September 2010 to April 2011. Nitrous oxide fluxes were measured on two replicates of four treatment. Treatment were; * Treatment 1 - nil applied, * Treatment 2 - urine @1000 kgN/ha applied to 25% of chamber base 23 Sept 2010, 21 Oct 2010, 10 Nov 2010 and 7 Dec 2010 * Treatment 3 - urine @ 1000 kg N/ha applied to 25% of the chamber base 23 Sept 2010, 21 Oct 2010, 10 Nov 2010 and 7 Dec 2010 with DCD @10 kg a.i./ha applied 23 Sept 2010 * Treatment 4 - urine @ 1000 kg N/ha applied to 25% of the chamber base 23 Sept 2010, 21 Oct 2010, 10 Nov 2010 and 7 Dec 2010 with DCD @ 10 kg a.i./ha applied each time (4) urine was applied. Nitrous oxide flux was measured in TWO replicates of all treatments. Measurement system used automated chambers linked to a Fourier Transform infrared spectometer for gas analysis. Daily flux (g N2O-N/ha/day) was calculated for each chamber. Soil water (surface 0-65 mm) and soil temperature (50mm) was measured continuously in each chamber base. Soil mineral N content of all treatment and replicates was measured (0-100 mm) routinely. Initial site soil physical and chemical status were defined. Standard meteorological information was collected for an on-site weather station. Nitrous oxide N2O Nitrification inhibitors Soil mineral nitrogen Dicyandiamide (DCD) Bovine urine Pasture Terang Auto Chambers Dairy Cattle VIC 0502 0799 anzsrc-for DemoDAIRY, Terang, Victoria, Australia 38 14 S, 142 55 E 142.92 142.92 -38.24 -38.24 1332290672437

Flux was measured using 8 automatic enclosure chambers (0.8m*0.8m*0.47m) with air drawn from each chamber in sequence at a flow rate of approximately 3 L/min and passed through a glass cell mounted in a Fourier Transform Infrared spectrometer (FTIR), all air was recycled to chambers. Instantaneous flux was calculated for one chamber every 30 minute period from an 18 minute closure period. Daily flux (g N2O-N/ha/day) was calculated from 6 measurement periods on each chamber each day as an arithmetic mean. Each chamber had to fixed bases with the chamber moved very week.Daily flux (g N2O-N/ha/day) was calculated from 6 measurement periods on each chamber each day as an arithmetic mean. Each chamber had to fixed bases with the chamber moved very week.

FTIR - standard Bomem FTLA2000 with KBr optics, multipass White cell and InSb detector, 32 m pathlength gas cell (approximate volume 4 L)

Surface soil water content (0-65 mm), two probes per chamber base, logged every 30 minutes (averag of 10 minute readings)

Theta-Probes Delta T MK2x

Soil temperature - logged every 30 minutes (averag of reading every 10 minutes)

Type-T thermocouples

Standard measurement weather station

MEA - Australia May 2011 - October 2011 Experimental design (4 treatments by 6 replicates) * Treatment 1 - nil applied, * Treatment 2 - urine @1000 kgN/ha applied to 25% of chamber base 23 Sept 2010, 21 Oct 2010, 10 Nov 2010 and 7 Dec 2010 * Treatment 3 - urine @ 1000 kg N/ha applied to 25% of the chamber base 23 Sept 2010, 21 Oct 2010, 10 Nov 2010 and 7 Dec 2010 with DCD @10 kg a.i./ha applied 23 Sept 2010 * Treatment 4 - urine @ 1000 kg N/ha applied to 25% of the chamber base 23 Sept 2010, 21 Oct 2010, 10 Nov 2010 and 7 Dec 2010 with DCD @ 10 kg a.i./ha applied each time (4) urine was applied. Nitrous oxide flux was measured in TWO replicates of all treatments. Weather - soil temperature, soil water, nitrous oxide flux, all chambers (8) Expt 2 part 2 NOTE: Flux measurements until the end of June we conducted using the automated system. Flux measurements for July to October we based on manual samplings, three days a week with missing days interpolated, samples were collected between 10 am and 12 noon (at this site this represents time of day when soil tempature are about daily average), samples were collected from enlcosed sealed chambers at T 0, 10, 20, 30 and 40 minutes after closure. Flux data is based on linear regression as no curvilinearity was evident in the dats sets. Terang data weather and flux data expt 2 part 2.csv column , ecogrid://knb/kelly.34.1 uid=datalibrarian,o=unaffiliated,dc=ecoinformatics,dc=org all uid=shcherbak,o=unaffiliated,dc=ecoinformatics,dc=org read uid=kelly,o=unaffiliated,dc=ecoinformatics,dc=org all Date DD-MM-YYY DD-MM-YYYY Minimum temperature Daily minimum air temperature - time period 9 am previous daty to 9 am date of record celsius natural Maximum temperature Daily maximum air temperature (period of measurement 9 am pervious day to 9 am date of record celsius natural Solar radiation (total) Total radiation (MJ/m^2) megajoulePersquaremeter natural Rainfall Daily rainfall (mm/day) (time period 9 am previous day to 9 am date of record) mm/day natural ETo (evaporanspiration) Potential evapotranspiration (mm/day) - calculated from weather station data using FAO 56 methodology mm/day natural Soil temperature (Treat 1, rep 1) Soil temperature (50 mm) celsius natural Soil water (Treat 1, rep 1) Surface soil water content (0-60 mm) - volumetric cubicmillimeterPercubicmillimeter natural Nitrous oxide flux (Treat 1, rep 1) Nitrous oxide flux (g N2O-N/ha/day) gramsNitrogenperHectareperDay natural Soil temperature (Treat 1, rep 2) Soil temperature (50 mm) celsius natural Soil water (Treat 1, rep 2) Surface soil water content (0-60 mm) - volumetric cubicmillimeterPercubicmillimeter natural Nitrous oxide flux (Treat 1, rep 2) Nitrous oxide flux (g N2O-N/ha/day) gramsNitrogenperHectareperDay natural Soil temperature (Treat 2, rep 1) Soil temperature (50 mm) celsius natural Soil water (Treat 2, rep 1) Surcafe soil water content (0-60 mm) - volumetric cubicmillimeterPercubicmillimeter natural Nitrous oxide flux (Treat 2, rep 1) Nitrous oxide flux (g N2O-N/ha/day) gramsNitrogenperHectareperDay natural Soil temperature (Treat 2, rep 2) Soil temperature (50 mm) celsius natural Soil water (Treat 2, rep 2) Surface soil water content (0-60 mm) - volumetric cubicmillimeterPercubicmillimeter natural Nitrous oxide flux (Treat 2, rep 2) Nitrous oxide flux (g N2O-N/ha/day) gramsNitrogenperHectareperDay natural Soil temperature (Treat 3, rep 1) Soil temperature (50 mm) celsius natural Soil water (Treat 3, rep 1) Surface soil water content (0-60 mm) - volumetric cubicmillimeterPercubicmillimeter natural Nitrous oxide flux (Treat 3, rep 1) Nitrous oxide flux (g N2O-N/ha/day) gramsNitrogenperHectareperDay natural Soil temperature (Treat 3, rep 2) Soil temperature (50 mm) celsius natural Soil water (Treat 3, rep 2) Surface soil water content (0-60 mm) - volumetric cubicmillimeterPercubicmillimeter natural Nitrous oxide flux (Treat 3, rep 2) Nitrous oxide flux (g N2O-N/ha/day) gramsNitrogenperHectareperDay natural Soil temperature (Treat 4, rep 1) Soil temperature (50 mm) celsius natural Soil water (Treat 4, rep 1) Surface soil water content (0-60 mm) - volumetric cubicmillimeterPercubicmillimeter natural Nitrous oxide flux (Treat 4, rep 1) Nitrous oxide flux (g N2O-N/ha/day) gramsNitrogenperHectareperDay natural Soil temperature (Treat 4, rep 2) Soil temperature (50 mm) celsius natural Soil water (Treat 4, rep 2) Surface soil water content (0-60 mm) - volumetric cubicmillimeterPercubicmillimeter natural Nitrous oxide flux (Treat 4, rep 2) Nitrous oxide flux (g N2O-N/ha/day) gramsNitrogenperHectareperDay natural Soil mineral N content - 4 treatments - Expt 2 part 2 Soil mineral N content (0-100 mm depth), expressed as NH4-N (N in ammonium form), and NO3-N (N in nitrate form) Terang soil mineral N expt 2.2.csv column , ecogrid://knb/kelly.35.2 uid=datalibrarian,o=unaffiliated,dc=ecoinformatics,dc=org all uid=shcherbak,o=unaffiliated,dc=ecoinformatics,dc=org read uid=kelly,o=unaffiliated,dc=ecoinformatics,dc=org all Date DD-MM-YYYY DD-MM-YYYY Treat 1, ammonium-N Soil ammonium-N (mg NH4-N/kg of soil) milligramPerkilogramofsoil natural Treat 2, ammonium-N Soil ammonium-N (mg NH4-N/kg of soil) milligramPerkilogramofsoil natural Treat 3, ammonium-N Soil ammonium-N (,g NH4-N/kg of soil) milligramPerkilogramofsoil natural Treat 4, ammonium-N Soil ammonium-N (mg NH4-N/kg of soil) milligramPerkilogramofsoil natural l.s.d. (P=0.05) Ammonium-N least significant differnce at P=0.05 n.s. = not significant milligramPerkilogramofsoil natural Treat 1, nitrate-N Soil nitrate-N (mg NO3-N/kg of soil) milligramPerkilogramofsoil natural Treat 2, nitrate-N Soil nitrate-N (mg NO3-N/kg of soil) milligramPerkilogramofsoil natural Treat 3, nitrate-N Soil nitrate-N (mg NO3-N/kg of soil) milligramPerkilogramofsoil natural Treat 4, nitrate-N Soil nitrate-N (mg NO3-N/kg of soil) milligramPerkilogramofsoil natural l.s.d. (P=0.05) Nitrate-N least significant difference at P=0.05 n.s. = not significant milligramPerkilogramofsoil natural megajoulePersquaremeter mm/day cubicmm/cubicmm Soil water content (volumetric) Nitrous oxide flux g N2O-N/ha/d mm^3/mm^3 Soil mineral nitrogen